This column comes from a laboratory that was shutting down operations. This column appears to be in good working order with a bed height of 450mm of Sephadex G-25 resin.
The Omni Chromatography Column (15 mm ID x 500 mm L) is a borosilicate glass column suitable for packing with Sephadex G-25 resin for size exclusion chromatography (specifically desalting and buffer exchange).
Column Specifications
Dimensions: 15 mm Inside Diameter (ID) x 500 mm Length (L).
Wetted Materials: Borosilicate glass, PTFE, PE (25𝜇m frits), FKM/FPM.
Pressure Rating: Suitable for low- to medium-pressure applications, up to 150 PSI.
Endpieces: Available with one adjustable and one fixed end type for flexible bed height management.
Temperature Range: 4°C to 40°C.
Sephadex G-25 Resin Characteristics
Purpose: Desalting, buffer exchange, and removal of small molecules (MW > 5000) from proteins and nucleic acids.
Matrix: Cross-linked dextran.
Particle Size: Medium (50–150𝜇m) or Fine (20–80𝜇m) are typically used for this application.
Operating Range: 4°C to 30°C–40°C.
This setup is ideal for rapid purification of large biomolecules from small molecules like salts, fluorescent dyes, or radioisotopes.
If you are in the Southern California area and would like to save on shipping costs, please contact us before purchase to coordinate local pickup!
This column comes from a laboratory that was shutting down operations. This column appears to be in good working order with a bed height of 450mm of Sephadex G-25 resin.
The Omni Chromatography Column (15 mm ID x 500 mm L) is a borosilicate glass column suitable for packing with Sephadex G-25 resin for size exclusion chromatography (specifically desalting and buffer exchange).
Column Specifications
Dimensions: 15 mm Inside Diameter (ID) x 500 mm Length (L).
Wetted Materials: Borosilicate glass, PTFE, PE (25𝜇m frits), FKM/FPM.
Pressure Rating: Suitable for low- to medium-pressure applications, up to 150 PSI.
Endpieces: Available with one adjustable and one fixed end type for flexible bed height management.
Temperature Range: 4°C to 40°C.
Sephadex G-25 Resin Characteristics
Purpose: Desalting, buffer exchange, and removal of small molecules (MW > 5000) from proteins and nucleic acids.
Matrix: Cross-linked dextran.
Particle Size: Medium (50–150𝜇m) or Fine (20–80𝜇m) are typically used for this application.
Operating Range: 4°C to 30°C–40°C.
This setup is ideal for rapid purification of large biomolecules from small molecules like salts, fluorescent dyes, or radioisotopes.
If you are in the Southern California area and would like to save on shipping costs, please contact us before purchase to coordinate local pickup!
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